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Journal: Molecular Therapy. Nucleic Acids
Article Title: Extracellular viral microRNAs as biomarkers of virus infection in human cells
doi: 10.1016/j.omtn.2024.102444
Figure Lengend Snippet: Viral exmiRs identify viral reactivation in cells (A–C) RT-dPCR quantification of cellular EBV genes EBNA1 (i) and BZLF1 (ii) at 6 and 24 h post-chemical induction of EBV-harboring B cells Jijoye (A), Raji (B), and Daudi (C). (D) Real-time RT-qPCR quantification of EBV miR-BHRF1-2-3p in spent media of non-induced and 24 h post-induction Jijoye (i), Raji (ii), and Daudi (iii) cells. (E–H) RT-dPCR quantification of cellular HHV-8 genes LANA and Rta at 6 and 24 h post-chemical induction of BC-1 (E) and BC-3 (G) cells. Real-time RT-qPCR quantification of HHV-8 miR-K12-10a-3p (i), miR-K12-10b (ii), and miR-K12-12-3p (iii) from spent media of non-induced and 24 h post-induction BC-1 (F) and BC-3 (H) cells. Data shown are from 4 biological repeats. Error bars represent the standard error of the mean.
Article Snippet: EBV type 2 was produced by treating the
Techniques: Quantitative RT-PCR
Journal: Journal of Virology
Article Title: CD21 (Complement Receptor 2) Is the Receptor for Epstein-Barr Virus Entry into T Cells
doi: 10.1128/JVI.00428-20
Figure Lengend Snippet: Comparison of gp350 amino acid sequences. The amino acid sequence (amino acids 1 to 504) of the gp350 protein of the EBV-1 strain Akata is shown. The known CD21 binding regions are underlined. The amino acid differences in gp350 of the EBV-2 strain Jijoye are in bold above the EBV-1 sequence.
Article Snippet: The EBV-2-positive Burkitt’s
Techniques: Comparison, Sequencing, Binding Assay